BelaxOmics

FAQs

1. How many samples do I need for a multiomics study?

The required number of samples depends on the study design, biological variability, and statistical power. As a general guideline:

• Pilot studies: 5-10 samples per group

• Exploratory studies: 15-30 samples per group

• Large-scale/clinical studies: 50 plus samples per group

We recommend performing a power calculation before finalizing sample numbers.

2. What types of samples can be used for multiomics?

Multiomics can be performed on a variety of biological samples, including:

• Plasma/serum

• Tissue (fresh, frozen, vivoPHIX, and FFPE for certain omics)

• Cells (cultured or primary)

• Urine, saliva, CSF, or stool (for metabolomics and lipidomics analysis)

3. What is the minimum sample quantity required?

Typical requirements (may vary depending on analysis):

• Proteomics: 

Tissue ~50-100 µg total protein (2-5 mg tissue)

Plasma/serum~20-50 µL

Cells ~ 2x106

• Metabolomics and Lipidomics:

Tissue: 10-50 mg

Plasma/serum: 50-100 µL

Stool: 50-100 mg

Urine: 2-5 mL

We will provide exact requirements during project planning.

4. How should I prepare and store my samples?

• Plasma/serum: Collect in EDTA or heparin tubes, spin within 2 hours, store at-80°C

• Tissue: Snap-freeze in liquid nitrogen, store at-80°C

• Urine/Stool/CSF/saliva: Store at-80°C immediately after collection

• Avoid repeated freeze-thaw cycles
  
  

5. Can I submit FFPE samples?

Yes, FFPE samples can be used for proteomics. Fresh or frozen samples are preferred whenever possible.

6. Do I need biological replicates?

Yes. Biological replicates are crucial for reliable results. At least 3-5 biological replicates per group are strongly recommended. Technical replicates

may be required for highly variable sample types.

7. Can I combine different omics layers on the same sample?

Yes. When sample amount is sufficient, we can perform multi-layer analysis (Proteomics + Metabolomics + Lipidomics) from the same

biological source, maximizing biological insight.

8. What quality control is done before analysis?

• Proteomics: Protein concentration and SDS-PAGE quality check

• Metabolomics/Lipidomics: Volume/weight verification and metabolite stability check.
  
  

9. What if my sample quantity is limited?

We offer low-input protocols for all three analyses (Proteomics, Metabolomics, and Lipidomics). Please contact us with details so we can suggest

the best workflow.

10. How should I ship my samples?

• Ship on dry ice for frozen samples.

• Use cold packs for short-term transport of stable samples.

• Clearly label all tubes and include a detailed sample sheet.